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1.
Rev. patol. trop ; 48(4): 249-252, 2019.
Article in English | LILACS | ID: biblio-1099600

ABSTRACT

In Brazil, about 99% of malaria cases occur in Brazil's Legal Amazon. In all other states malaria has been noted but with imported cases. Here we describe an autochthonous case of malaria in Petropolis city, Rio de Janeiro. The clinical symptoms and epidemiological aspects were compatible with malaria and the presence of Plasmodium was confirmed through molecular diagnostic testing performed on a blood sample from a local resident after an ecological hike in the Atlantic Forest on the outskirts of town. After treatment with chloroquine and primaquine, the symptoms ceased. This city was regarded malaria free for many years although it still presents the malaria vectors. Considering that Plasmodium sp. is an important cause of morbidity and mortality worldwide, this clinical case serves as an epidemiological alert.


Subject(s)
Epidemiologic Factors , Malaria, Vivax , Malaria
2.
Pesqui. vet. bras ; 37(6): 593-597, jun. 2017. tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-895451

ABSTRACT

Talassemias e hemoglobinopatias são condições hereditárias encontradas em humanos de todo o mundo. Em medicina veterinária, o polimorfismo de hemoglobinas tem sido estudado em animais de produção, mas não existem relatos de hemoglobinopatias em cães, e os estudos envolvendo o polimorfismo de hemoglobinas nesta espécie são escassos. Com o objetivo de pesquisar variantes da hemoglobina em cães, foram coletadas amostras de sangue de 202 caninos de variadas raças, sendo 130 portadores de anemia crônica (Grupo Experimental) e 72 animais clinicamente saudáveis (Grupo Controle). Estas amostras foram submetidas à eletroforese alcalina de hemoglobinas, que permitiu a separação e quantificação das frações de hemoglobina por densitometria, e posteriormente submetidas à eletroforese de hemoglobinas em meio ácido, técnica utilizada em medicina humana para a separação de frações de hemoglobinas variantes que não se diferenciam em meio alcalino. O eritrograma e índices hematimétricos foram obtidos concomitantemente. Os métodos utilizados demonstraram que a HbA é o maior componente da hemoglobina canina, e que uma pequena quantidade de HbA2 pode ser detectada em uma parcela dos animais avaliados, sendo que a maioria dos caninos apresentava exclusivamente HbA em sua composição. Concluiu-se que a presença ou ausência de HbA2 não interfere nos índices hematimétricos dos animais avaliados, e que quando comparadas as hemoglobinas dos grupos Experimental e Controle, não são observadas diferenças na distribuição das frações destas, além de não serem observadas hemoglobinas variantes nos caninos avaliados.(AU)


Thalassemias and hemoglobinopathies are hereditary conditions found in humans throughout the world. In veterinary medicine, hemoglobin polymorphism has been studied in production animals, but there are no reports of hemoglobinopathies in dogs, and studies involving hemoglobin polymorphism in this species are scarce. In order to search for hemoglobin variants in dogs, blood samples were collected from 202 dogs of various breeds, being 130 patients with chronic anemia (Experimental Group) and 72 clinically healthy animals (Control Group). These samples were subjected to alkaline electrophoresis of hemoglobin, which permitted separation and quantification of hemoglobin fractions by densitometry, and then subjected to hemoglobin electrophoresis in an acid medium, a technique used in human medicine for the separation of variant fractions of hemoglobin that do not differentiate in an alkaline medium. The erythrogram and RBC indices were obtained concurrently. The methods demonstrated that HbA is the major component of canine hemoglobin, and a small amount of HbA2 can be detected in some of the evaluated animals, and most dogs showed only HbA in its composition. It was concluded that the presence or absence of HbA2 does not interfere with RBC indices of evaluated animals, and the comparison between the hemoglobin of Experimental and Control groups showed no differences in fractions distribution between them, and there was no hemoglobin variants in evaluated canines.(AU)


Subject(s)
Animals , Dogs , Hemoglobins , Hemoglobinopathies/veterinary , Hemoglobinopathies/epidemiology , Anemia/veterinary , Electrophoresis/veterinary
3.
Mem. Inst. Oswaldo Cruz ; 92(4): 477-81, July-Aug. 1997. mapas, tab
Article in English | LILACS | ID: lil-193149

ABSTRACT

A study on tick-borne rickettsiosis was developed in the county of Santa Cruz do Escalvado, State of Minas Gerais, Brazil, where a clinical case of the disease, confirmed by necropsy, had been reported. Of the 1,254 ticks collected 1,061 belonged to the Amblyomma genus, 57 to the Rhipicephalus sanguineus species, 81 to Boophilus microplus, and 46 to Anocentor nitens. The hemolymph test associated with Gimenez staining showed that 18 of the 221 A. cajennense specimens, 1 of the 16 R. sanguineous, 1 of the 22 B. microplus, 3 of the A. nitens, and 1 of the A. ovale contanined rickettsia-like microorganisms. Only 3 A. cajennense ticks were positive under direct immunofluorescence. A. cajennense was the only species found on humans.


Subject(s)
Animals , Rocky Mountain Spotted Fever/epidemiology , Ticks/classification , Ticks/parasitology , Rickettsia rickettsii , Rickettsiaceae Infections/epidemiology
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